Résumé de section

    • The Molecular Biology and Genetic Engineering course covers the study of the molecular mechanisms governing cellular function, including DNA replication, transcription, protein translation, and genetic regulation mechanisms. The genetic engineering section addresses techniques for manipulating genetic material, such as PCR, gene cloning, genome editing, and the application of these methods in fields like medicine, agriculture, and biotechnology. The course also explores the ethical implications and industrial applications of modern biotechnology.

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      • University: Mohamed Khider University of Biskra

      • Faculty: Faculty of Nature and Life Sciences and Earth and Universe Sciences

      • Department: Natural and Life Sciences

      • Target Audience: 3rd Year (Licence), Specialty: Microbiology

      • Course Title: Molecular Biology and Genetic Engineering

      • Credits: 5

      • Coefficient: 3

      • Duration: 13 weeks

      • Schedule and Location:

        • Lectures: 

          Monday at 9:30 AM in S 6 

          Tuesday at 11:00 AM in GS4

      • Instructor: Dr. Samia CHARIFI

      • Contact: Samia.charifi@univ-biskra.dz

      • Availability: Office hours on Thursday from 11:00 AM to 12:30 PM, Office 07 at the Central Library

      • Evaluation Method:

        • Continuous Assessment (40%): 8 points for Quizzes (2 Quizzes) + 2 points for Class Participation Final Exam (60%): 20 pointsInformation Animation

          • Forum Responses: All questions related to the course must be posted on the dedicated Moodle forum; I commit to answering questions within 24 to 48 hours.

          • By Email: I commit to responding by email within 48 hours of receiving the message (in case of platform access issues).Texting GIFs - Get the best gif on GIFER

        • You must acquire the necessary basic concepts in the following fields before starting the course:

          • General Biology:

            • Understanding the cell as the basic unit of life, including cellular organelles (nucleus, mitochondria, ribosomes, etc.).

            • Basic concepts of mitosis and meiosis.

            • Understanding the structure of DNA, genes, chromosomes, and mechanisms of heredity.

          • Biochemistry:

            • Knowledge of metabolic pathways such as glycolysis and the Krebs cycle.

          • Immunology:

            • Differentiation and functions of immune cells (B and T lymphocytes, macrophages).

            • Role of antibodies in antigen recognition and mechanisms of innate and adaptive immune responses.

          • Genetics:

            • Basic knowledge of gene transmission, mutations, and principles of inheritance.Pré-requis - Convergo Formations

        • This prerequisite test is intended for 3-Licence’s students in Microbiology.

          The prerequisite test is available starting from the first week and is accessible with no time limit so that you can retake it. If your score is insufficient (below 50%), you will be directed to a self-paced, self-study course to follow at your own speed and progress.

          If your score is insufficient, please click on the following link:

          Microsoft Word - 2020-2021-LE- Biochimie Moléculaire-12-Réplication-V1.docx

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        • Objectives of the Molecular Biology and Genetic Engineering course:

          • Memorize basic concepts, such as DNA structure, transcription, translation, as well as various genetic engineering techniques.

          • Understand mechanisms of genetic regulation, mutations, and be capable of explaining fundamental biological processes such as DNA replication or genetic modification.

          • Be capable of analyzing experimental results in molecular biology, detecting potential errors, and suggesting improvements.

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    • Part I: Molecular Biology

      1. Gene Expression

      2. Regulation of Gene Expression

      3. Basic Techniques in Molecular Biology

      Part II: Genetic Engineering

      1. In Vivo Cloning:

        • 1.1. Required elements for cloning

        • 1.2. Steps of cloning

      2. Recombinant DNA Technology

    • By the end of this chapter, students will be able to:

      • Define gene expression and explain its role in protein synthesis.
      • Describe the main stages of gene expression: transcription and translation.
      • Explain the process of transcription and the role of RNA polymerase.
      • Identify the template and coding DNA strands and determine the corresponding mRNA sequence.
      • Describe the main steps of transcription: initiation, elongation, and termination.
      • Explain mRNA processing in eukaryotic cells, including 5′ capping, polyadenylation, and splicing.
      • Explain the process of translation and the roles of mRNA, tRNA, and ribosomes.
      • Use the genetic code to determine the amino acid sequence of a protein.
      • Apply their knowledge to solve problems and interpret molecular biology exercises.
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